Patent · US Expired

Chimeric RNA molecules generated by trans-splicing

US6013487A · kind A · utility

62Cited by
7References
34Claims
0Family size

Inventor

Key dates

Filing dateDec 13, 1996
Grant dateJan 11, 2000
Priority date
Expiry dateDec 13, 2016

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2840/445
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

The molecules and methods of the present invention provide a means for in vivo production of a therapeutic molecule in a selected subset of cells. The pre-therapeutic molecules of the invention are substrates for a trans-splicing reaction between the pre-therapeutic molecules and a pre-mRNA which is uniquely expressed in the specific target cells. The in vivo trans-splicing reaction provides an active therapeutic RNA which is functional as RNA or encodes a protein to be expressed in the target cells. The expression product of the mRNA is a protein of therapeutic value to the cell or a toxin which causes killing of the specific cells.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.