Breast tumor cells for study of nonionizing radiation effects
US6013520A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 20, 1998 |
| Grant date | Jan 11, 2000 |
| Priority date | — |
| Expiry date | Aug 20, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/57415
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The cell line EMT-6 are transformed with the chromosomal insertion of the plasmid pSV.sub.2 neoNR10.sub.1, ATCC No. 69617. The transformed cells, EMT-6/pSV.sub.2 neoNR10.sub.1, produce diazoluminomelanin (DALM) intracellularly when provided with nitrate, luminol and 3-amino-L-tyrosine.multidot.HCl (3AT). The modified cells can be used to study mechanisms for radiofrequency and light radiation interactions with breast tumor cells in vitro and in mice. The effects of drugs, hormones, and cytokines that affect the expression of nitric oxide synthase and its activity can also be studied to understand the effects of these materials on breast tumor cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.