Restriction enzyme screen for differentiating porcine reproductive and respiratory syndrome virus strains

US6015663A · kind A · utility

Assignee

• The United States of America, as represented by the Secretary of Agriculture · US

Inventors

• Ronald D. Wesley · Ames, US
• Deborah F. Clouser · Ames, US
• William L. Mengeling · Ames, US
• Vladimir G. Andreyev · Vladimir, RU
• Ann Vorwald · Ames, US
• Kelly M. Lager · Nevada, US

Key dates

• Filing date: Mar 1, 1996
• Grant date: Jan 18, 2000
• Expiry date: Mar 1, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/10022
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A test based on restriction enzyme analysis identifies and differentiates strains of porcine reproductive and respiratory syndrome virus (PRRSV). Amplified cDNA from the ORF 5 region of the viral genome has been targeted for identification of unique restriction sites that allow for the differentiation of the vaccine strain from field strains, and for differentiation of field strains from each other through the use of selected restriction enzymes. This assay is useful for both clinical diagnosis of PRRSV field strains in vaccinated pigs as well as for epidemiological studies in the evaluation of the source and transmission of PRRS field viruses.