Rapid immunoassay for cariogenic bacteria
US6015681A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 12, 1996 |
| Grant date | Jan 18, 2000 |
| Priority date | — |
| Expiry date | Dec 12, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An assay method and kit for detecting specific oral cariogenic bacteria, ., mutans streptococci, Lactobacillus sp. and Actinomyces sp., separately or in combination, comprising gathering a sample suspected of containing cariogenic bacteria; treating the sample with a stripping buffer to remove host antibodies from bacteria present in the sample; retaining the treated bacteria on a blocked solid phase substrate; reacting the retained bacteria with a primary antibody specific for the desired cariogenic bacteria; reacting the primary antibody with a conjugated label producing a detectable signal; and detecting the signal whereby the presence of the desired cariogenic bacteria is determined in the sample. The device for conducting these assays is a frame or support which holds a solid substrate capable of retaining the bacteria of interest while permitting drainage of other materials or fluids away from the retained bacteria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.