Dry analytical element for acetaminophen assay
US6015683A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 15, 1992 |
| Grant date | Jan 18, 2000 |
| Priority date | — |
| Expiry date | Jul 15, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/815
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A spectrophotometric assay for the detection of acetaminophen in aqueous fluids can be carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; a second enzyme, ascorbic acid oxidase, to oxidize the p-aminophenol so that it couples to a water soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.