Characterization of individual polymer molecules based on monomer-interface interactions

US6015714A · kind A · utility

Assignees

• Government of the United States of America, as represented by the Secretary of Commerce · US
• The Regents of the University of California · US

Inventors

• Richard Baldarelli · Bar Harbor, US
• Daniel Branton · Lexington, US
• George M. Church · Brookline, US
• David W. Deamer · Santa Cruz, US
• Mark A. Akeson · Santa Cruz, US
• John J. Kasianowicz · Gaithersburg, US

Key dates

• Filing date: Jun 16, 1998
• Grant date: Jan 18, 2000
• Expiry date: Jun 16, 2018

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S977/953
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for sequencing a nucleic acid polymer by (1) providing two separate, adjacent pools of a medium and an interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage from one pool to the other pool of only one nucleic acid polymer at a time; (2) placing the nucleic acid polymer to be sequenced in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the nucleic acid polymer passes through the channel so as to sequence the nucleic acid polymer.