Detection of endotoxin levels in liposomes, lipid bilayers and lipid complexes
US6015716A · kind A · utility
2Cited by
8References
20Claims
0Family size
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Key dates
| Filing date | Jul 12, 1996 |
| Grant date | Jan 18, 2000 |
| Priority date | — |
| Expiry date | Jul 12, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/104165
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Endotoxin incorporated into liposomes, lipid bilayers or lipid complexes can be detected by combining an aqueous suspension of the liposomes, lipid complexes or lipid bilayers with a suitable detergent. Preferable detergents, e.g., Lubrol-PX.TM. or a polyoxyethylene ether, solubilize the lipid bilayers, liposomes or lipid complexes at acceptable lipid concentrations, forming micelles therefrom which contain lipid bilayer, liposome or lipid complex lipid, detergent and endotoxin, should it be present. The micelles are then assayed for the presence of endotoxin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.