Methods for electronic stringency control for molecular biological analysis and diagnostics
US6017696A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 7, 1994 |
| Grant date | Jan 25, 2000 |
| Priority date | — |
| Expiry date | Jul 7, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25125
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.