Protease-containing cleaning compositions

US6017871A · kind A · utility

Assignee

• The Procter & Gamble Company · US

Inventors

• Andre Baeck · Bonheiden, BE
• Chanchal Kumar Ghosh · Sharonville, US
• Thomas P. Graycar · Pacifica, US
• Richard R. Bott · Burlingame, US
• Lori J. Wilson · Millbrae, US
• Philip Frederick Brode, III · Cincinnati, US
• Bobby Lee Barnett · Cincinnati, US
• Donn Nelton Rubingh · Cincinnati, US

Key dates

• Filing date: Jul 22, 1997
• Grant date: Jan 25, 2000
• Expiry date: Jul 22, 2017

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K2800/86
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to cleaning compositions comprising protease enzyme which is a carbonyl hydrolase variant having an amino acid sequence not found in nature, which is derived by replacement of a plurality of amino acid residues of a precursor carbonyl hydrolase with different amino acids, wherein said plurality of amino acid residues replaced in the precursor enzyme correspond to position +76 in combination with one or more of the following residues: +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265, and/or +274, where the numbered positions corresponds to naturally-occurring subtilisin from Bacillus amyloliquefaciens or to equivalent amino acid residues in other carbonyl hydrolases or subtilisins (such as Bacillus lentus subtilisin).