Nucleic acids encoding CR1 fusion protein, vector, transfected cell and expression
US6020155A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 5, 1995 |
| Grant date | Feb 1, 2000 |
| Priority date | — |
| Expiry date | Jun 5, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/158
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for producing a complementary DNA (cDNA) library enriched in ligand-inducible genes of a cell is described. The method includes activating a cellular ligand receptor for a predetermined period of time in the presence of labelled RNA precursors and a substance which enhances the level of RNA in the cell. The RNA precursors are incorporated into the RNA synthesized by the cell in response to receptor activation. The labelled RNA is separated from unlabelled RNA and used to prepare cDNA. The cDNA is cloned into host cells to provide a cDNA library of cDNA-containing clones, which is then screened for clones containing ligand-induced genes. Also described are several novel ligand-induced genes identified using the method of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.