C. antarctica lipase and lipase variants
US6020180A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 8, 1998 |
| Grant date | Feb 1, 2000 |
| Priority date | — |
| Expiry date | Jul 8, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2299/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A DNA sequence encoding a lipase having the amino acid sequence shown in SEQ ID NO:2. The lipase has a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule and, located in a critical position of a lipid contact zone of the lipase structure, an amino acid residue different from an aromatic amino acid residue, which amino acid residue interacts with a lipid substrate at or during hydrolysis, in which lipase variant of the amino acid residue has been replaced by an aromatic amino acid residue so as to confer to the variant an increased specific activity as compared to that of the parent lipase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.