Device and process for isolating nucleic acids from cell suspensions

US6020186A · kind A · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Karsten Henco · Erkrath, DE
• Metin Colpan · Essen, DE
• Petra Feuser · Cologne, DE

Key dates

• Filing date: May 16, 1995
• Grant date: Feb 1, 2000
• Expiry date: May 16, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A device and a process for isolating nucleic acids by lysing intact cells and removing nucleic acids emerging from the lysed cells by the following steps: PA1 a) the cells are immobilized in a porous matrix, with the size of matrix voids being in the range of the type of cell to be lysed; PA1 b) the cells are lysed; PA1 c) the nucleic acids are fixated on the matrix surface, and subsequently PA1 d) are eluted.