Process for transforming Gramineae and the products thereof
US6020539A · kind A · utility
Inventors
Key dates
| Filing date | Jun 27, 1994 |
| Grant date | Feb 1, 2000 |
| Priority date | — |
| Expiry date | Jun 27, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8205
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir.sup.+ Agrobacterium tumefaciens. Also, this same method wherein the vir.sup.+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir.sup.+ Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir.sup.+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, ther…
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