Expression of DNA sequences derived from nocardioform microorganisms
US6022703A · kind A · utility
4Cited by
6References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 21, 1993 |
| Grant date | Feb 8, 2000 |
| Priority date | — |
| Expiry date | Jun 21, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/76
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A cloning vector contains a DNA sequence derived from a Nocardioform microorganism, and is capable of expressing the DNA sequence in a host microorganism. Recombinant cholesterol oxidase is produced by a host microbial cell transformed with a cloning vector containing cholesterol oxidase encoding DNA derived from a Nocardioform microorganism. A method for determining cholesterol oxidase in a sample of fluid from a human includes contacting the sample with the Nocardioform microorganism derived recombinant cholesterol oxidase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.