Method for the specific coupling of the cap of the 5' end of an mRNA fragment and preparation of mRNA and complete cDNA
US6022715A · kind A · utility
91Cited by
3References
41Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 31, 1997 |
| Grant date | Feb 8, 2000 |
| Priority date | — |
| Expiry date | Oct 31, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1096
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for the specific coupling of the cap of the 5' end of a eukaryotic mRNA fragment by a molecule functionalized by an amine functional group. The present invention also provides a method for isolating mRNA 5' end and a method for the preparation of single-stranded cDNA end complementary to the 5' end of mRNA and of double-stranded cDNA corresponding to the mRNA 5' end. The present invention also provides a method for isolating whole-length cDNA corresponding to the entire mRNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.