Hybrid molecules having translocation region and cell-binding region
US6022950A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 7, 1995 |
| Grant date | Feb 8, 2000 |
| Priority date | — |
| Expiry date | Jun 7, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16022
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a hybrid molecule comprising a first part, a second part, and a third part connected by covalent bonds, PA1 (a) wherein said first part comprises a portion of the binding domain of a cell-binding polypeptide ligand effective to cause said hybrid protein to bind to a cell of an animal; PA1 (b) wherein said second part comprises a portion of a translocation domain of naturally occurring protein which translocates said third part across the cytoplasmic membrane into the cytosol of the cell; and PA1 (c) wherein said third part comprises a chemical entity to be introduced into the cell, wherein each of said first part and said third part is non-native with respect to said naturally occurring protein, and further wherein said covalent bond connecting said second part and said third part is a cleavable bond, provided that when said second part comprises a portion of a translocation domain of Pseudomonas exotoxin, said third part is not a polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.