Method for high resolution liquid chromatographic separation of polynucleotides
US6024878A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 30, 1998 |
| Grant date | Feb 15, 2000 |
| Priority date | — |
| Expiry date | Oct 30, 2018 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D15/366
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Mixtures of dsDNA fragments are separated by Matched Ion Polynucleotide Chromatography (MIPC) using an isocratic mobile phase to elute polynucleic acid from an MIPC column. The use of isocratic elution conditions provides a marked improvement in the separation of dsDNA fragments compared to gradient elution conditions. Isocratic elution can also be used to effect an improved separation of heteroduplex and homoduplex mixtures when the chromatography is performed under partially denaturing conditions. In addition, dsDNA fragments are bound to the stationary phase under isocratic conditions until a solvent concentration is reached which releases fragments of a particular base pair length range. This separation process is different from the equilibrium partitioning process observed under gradient elution conditions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.