Patent · US Expired

Facile method for identifying regulated promoters

US6025131A · kind A · utility

2Cited by

1References

11Claims

0Family size

Assignee

E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

Robert A. Larossa · Chadds Ford, US
Tina K. Van Dyk · Wilmington, US

Key dates

Filing date	Oct 23, 1996
Grant date	Feb 15, 2000
Priority date	—
Expiry date	Oct 23, 2016

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6897
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A new method for the identification of useful promoters is disclosed. The method is capable of identifying bacterial promoters sensitive to a particular cellular insult and may be modified to identify promoters sensitive to herbicides and crop protection chemicals. Constructs comprising promoters upstream of a luminescent reporter genes are placed in transformed hosts. Transformants grown in liquid media to a predetermined growth stage and contacted with a cellular insult are assessed for regulatory region activity by measurement of the resulting change in bioluminesence. The method is able to identify promoters undetectable by standard methods.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.