Promoter-sequestered oligonucleoside and method of use
US6025133A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 30, 1996 |
| Grant date | Feb 15, 2000 |
| Priority date | — |
| Expiry date | Dec 30, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention features "promoter-sequestered" oligonucleosides and the use of such oligonucleosides to achieve "target-triggered" amplification. A promoter-sequestered oligonucleoside contains a contiguous nucleic acid sequence which forms a stem-loop structure in the absence of a target sequence. The stem-loop structure contains a single-stranded loop region and a double-stranded stem region. The single-stranded loop contains all, or a portion of, an RNA polymerase promoter sequence. The stem is produced from two substantially complementary nucleic acid sequences able to form an intramolecular hybrid. The secondary structure of the stem decreases the accessibility of the loop promoter sequence to form a functional double-stranded promoter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.