Membrane-permeable constructs for transport across a lipid membrane

US6025140A · kind A · utility

Assignee

• PerSeptive Biosystems Inc. · US

Inventors

• Ulo Langel · Tartu, EE
• Tamas Bartfai · Stockholm, SE
• Margus Pooga · Tartu, EE
• Andres Valkna · Tartu, EE
• Kulliki Saar · Lidingö, SE
• Mattias Hallbrink · Stockholm, SE

Key dates

• Filing date: Jul 16, 1998
• Grant date: Feb 15, 2000
• Expiry date: Jul 16, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/3513
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Constructs of peptides and nucleic acid analogs conjugated together for transport across a lipid membrane and for delivery into interactive contact with intracellular polynucleotides are disclosed. Transport is effected through at least the exterior membrane of a cell, and most likely also through the walls of subcellular structures separated from the cytosol by lipid membranes, including the nucleus, mitochondria, ribosomes, etc. Peptide nucleic acid (PNA) analog sequences conjugated through a labile disulfide bond to transporting peptides, are intracellulary cleaved, and target mRNA (antigene) or dsDNA (antisense).