Patent · US Expired

Temperature-sensitive plasmid

US6025190A · kind A · utility

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1References

19Claims

0Family size

Assignee

INSTITUT NATIONAL DE LA RECHERCHE AGRONOMQIUE · FR

Inventors

Alexandra Gruss · Orsay, FR
Emmanuelle Maguin · Montrouge, FR

Key dates

Filing date	Dec 27, 1994
Grant date	Feb 15, 2000
Priority date	—
Expiry date	Dec 27, 2014

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/902
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A bacterial vector plasmid of the type having an efficient replication origin in Gram-positive bacteria. The plasmid is characterized by having at least one marker gene which expresses itself in a bacterial host strain, an efficient replication system which is thermosensitive based on a temperature compatible with the viability of the host strain, and in that the temperature of replication inhibition is below or equal to approximately 37.degree. C. The invention also concerns bacteria containing the plasmid and a process for inactivating a gene in the chromosome of a bacterium.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.