Glycosylated protein-liposome conjugates and methods for their preparation
US6027726A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 29, 1995 |
| Grant date | Feb 22, 2000 |
| Priority date | — |
| Expiry date | Sep 29, 2015 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K47/6913
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides glycosylated protein-liposome compositions which are useful for the targeted delivery of a therapeutic agent. The compositions contain an oxidized protein, typically an antibody, which is covalently attached to a lipid by means of a crosslinking agent having an acid hydrazide functionality on one terminus and a sulfhydryl functionality on the other terminus. The lipid is present in a liposome formulation. Methods for preparing the compositions are also provided. In the methods, a glycosylated protein is first oxidized then reacted with a lining group having an acid hydrazide on one end and a sulfhydryl or protected sulfhydryl group on the other end. The resultant modified protein is then reacted with a liposome formulation of a lipid having a sulfhydryl reactive functional group to covalently attach the protein to the liposome.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.