Patent · US Expired

Systems and methods for the rapid isolation of nucleic acids

US6027750A · kind A · utility

9Cited by
4References
18Claims
0Family size

Inventors

Key dates

Filing dateJan 25, 1996
Grant dateFeb 22, 2000
Priority date—
Expiry dateJan 25, 2016

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N15/1006
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention contemplates a system for rapidly isolating nucleic acids. The system comprises an insoluble silica matrix and a buffered aqueous salt solution containing salt at a concentration of at least 3 molar and a buffering agent at a concentration sufficient to provide a buffering capacity corresponding to that which either tris(hydroxymethyl)aminomethane or phosphate ion at a concentration of 0.1 to 1 molar would provide in the solution. Methods of using the system are also contemplated.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.