Chromatographic method for mutation detection using mutation site specifically acting enzymes and chemicals
US6027898A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 18, 1998 |
| Grant date | Feb 22, 2000 |
| Priority date | — |
| Expiry date | Aug 18, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/137
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for analyzing a sample of double stranded DNA to determine the presence of a mutation therein comprises contacting the sample with a mutation site binding reagent, and chromatographically separating and detecting the product. The chromatographic separation can be performed using Matched Ion Polynucleotide Chromatography, size exclusion chromatography, ion exchange chromatography, or reverse phase chromatography. The mutation site binding reagent can be an enzyme or a non-proteinaceous chemical reagent. In one embodiment, a mutation site binding reagent binds to the site of mutation and alters the chromatographic retention time. In another embodiment, a mutation site binding reagent cleaves at the site of mutation, resulting in an increase in the number of fragments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.