Methods and tools for transformation of eukaryotic algae

US6027900A · kind A · utility

Assignees

• Carnegie Institution of Washington · US
• Martek Biosciences Corporation · US

Inventors

• F. C. Thomas Allnutt · Bethesda, US
• David J. Kyle · Davis, US
• Arthur Grossman · Stanford, US
• Kirk R. Apt · Columbia, US

Key dates

• Filing date: Oct 9, 1998
• Grant date: Feb 22, 2000
• Expiry date: Oct 9, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/65
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Genetic fusions for use in genetic engineering of eukaryotic algae employ a promoter from a light harvesting protein fused to a protein of interest. The fusions can be introduced and selected using an antibiotic resistance determinant. One gene useful for such selection is the sh ble gene encoding a bleomycin binding protein.