System for protein expression and secretion especially in corynebacteria
US6027920A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 1995 |
| Grant date | Feb 22, 2000 |
| Priority date | — |
| Expiry date | Jul 31, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A DNA cassette for expression and secretion of a given amino acid, polypeptide or protein in a host strain of corynebacterium, including a sequence which encodes the amino acid, polypeptide or protein, and a region of chromosomal or plasmid DNA, wherein the sequence is situated in the region of chromosomal or plasmid DNA such that the sequence is transcribed with, at the sequence's 5' end, at least one portion of a sequence encoding the signal sequence of the protein PS1 or PS2, the at least one portion ensuring the secretion of the amino acid, polypeptide or protein after translation when the DNA cassette is incorporated into the host strain of corynebacterium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.