Genetically engineered microorganisms and method for producing 4-hydroxybenzoic acid
US6030819A · kind A · utility
8Cited by
1References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 28, 1998 |
| Grant date | Feb 29, 2000 |
| Priority date | — |
| Expiry date | Sep 28, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention pertains to a method for economical biofermentative production of 4-hydroxybenzoic acid (PHB) using genetically engineered E. coli. According to the invention, a plasmid is provided which controls the overexpression of chorismate pyruvate lyase, the bacterial enzyme which catalyzes the production of PHB from chorismate. Mutant E. coli selected with a unique two-step screening assay to overproduce chorismate have been transformed with this plasmid, providing a biocatalyst that efficiently converts glucose to PHB.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.