Method for suppressing nonspecific hybridization in primer extension method
US6033851A · kind A · utility
18Cited by
12References
28Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 30, 1997 |
| Grant date | Mar 7, 2000 |
| Priority date | — |
| Expiry date | May 30, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An object of the present invention is to suppress nonspecific extension reaction of the primer in the primer extension method. The primer extension reaction to form a nucleic acid strand complementary to a nucleic acid template strand with the use of a primer according to the present invention is characterized in that the reaction between the primer and the template strand is carried out in the presence of a nucleic acid or a derivative thereof which is complementary to said primer and has an affinity for said primer is equivalent to or less than that of said primer for the nucleic acid template strand.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.