Quantitative PCR using blocking oligonucleotides
US6033854A · kind A · utility
72Cited by
12References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 27, 1998 |
| Grant date | Mar 7, 2000 |
| Priority date | — |
| Expiry date | Jan 27, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6851
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for determining the number of copies of a target nucleic acid relative to the number of copies of a reference nucleic acid in a biological specimen. The method includes amplifying the target nucleic acid, measuring the amplification of the target nucleic acid, amplifying the reference nucleic acid, measuring the amplification of the target nucleic acid, and comparing the amplification of the target nucleic acid sequence to the amplification of the reference nucleic acid sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.