Method of expressing and secreting soluble extracellular domains of human gonadotropin hormone receptors

US6033903A · kind A · utility

Assignee

• APPLIED RESEARCH SYSTEMS ARS HOLDING N.V.

Inventors

• William Sisk · Kensington, US
• Shirley Vui Yen Cheng · Boston, US
• David Buckler · Mount Olive, US
• Holly Prentice · Carlisle, US

Key dates

• Filing date: Jun 2, 1995
• Grant date: Mar 7, 2000
• Expiry date: Jun 2, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/14143
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The extracellular domain, or fragment thereof, of a gonadotropin glycoprotein hormone receptor is expressed and secreted in a soluble and functionally hormone-binding form. A recombinant baculovirus transfer vector is constructed to include a gene segment encoding the extracellular domain, or fragment thereof, of the glycoprotein hormone receptor joined in frame with a gene segment encoding a baculovirus signal peptide and operably linked to a baculovirus promoter. Recombinant baculovirus generated by transfection or co-transfection of insect cells are then used to infect insect host cells for the expression and secretion of soluble receptor.