Wavelength-shifting probes and primers and their use in assays and kits

US6037130A · kind A · utility

Assignee

• The Public Health Institute of the City of New York, Inc. · US

Inventors

• Sanjay Tyagi · New York, US
• Fred R. Kramer · Yonkers, US
• Salvatore A. E. Marras · Roselle Park, US

Key dates

• Filing date: Jul 28, 1998
• Grant date: Mar 14, 2000
• Expiry date: Jul 28, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6818
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Hairpin-forming oligonucleotide probes and primers triple-labeled with a pair of florophores, a shorter wavelength harvester and a longer wavelength emitter, and a quencher. When the probes and primers are stimulated by light that excites the harvester, opening causes an increase in fluorescence by the emitter, while fluorescence from the harvester is continually suppressed. The probes and primers may be used for detection of nucleic acid targets in assays, including amplification assays. Assay kits are provided.