Patent · US Expired

Polypeptide of N-acetylglucosamine-6-O-sulfotransferase and DNA encoding the same

US6037159A · kind A · utility

10Cited by
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13Claims
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Assignee

Inventors

Key dates

Filing dateMar 5, 1999
Grant dateMar 14, 2000
Priority date
Expiry dateMar 5, 2019

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12P19/04
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Apolypeptide of N-acetylglucosamine-6-O-sulfotransferase and a DNA encoding the peptide are provided. The polypeptide is (a) or (b) below: PA1 (a) a polypeptide consisting of an amino acid sequence represented by SEQ ID NO: 2; or PA1 (b) a polypeptide which comprises an amino acid sequence including substitution, deletion, insertion or transposition of one or few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a sulfate group from a sulfate group donor to a hydroxyl group at 6 position of an N-acetylglucosamine residue located at a non-reducing end of an oligosaccharide represented by the formula I: EQU GlcNAc.beta.1-3Gal.beta.1-4GlcNAc (I) PA1 wherein GlcNAc represents an N-acetylglucosamine residue, Gal represents a galactose residue, .beta.1-3 represents a .beta.1-3 glycosidic linkage, and .beta.1-4 represents a .beta.1-4 glycosidic linkage.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.