Preparation of serum-free suspensions of human hematopoietic cells or precursor cells
US6037174A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 19, 1997 |
| Grant date | Mar 14, 2000 |
| Priority date | — |
| Expiry date | Nov 19, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/948
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Provided are serum-free, animal protein-free media formulations to be used in conjunction with hematopoietic growth factors for the in vitro growth of human neutrophil and megakaryocyte precursors. The medium contains a base medium, corticosteroid, transferrin, insulin, cholesterol, ethanolamine, and human albumin. Also provided are methods for preparing serum-free, animal protein-free suspensions of human hematopoietic precursor cells wherein the cellular component contains at least about 16% neutrophil precursors and at least about 1% megakaryocyte precursors. Serum-free, animal protein-free suspensions of human hematopoietic cells are provided wherein the cellular component comprises at least about 30%, preferably greater than 60% neutrophil precursors. The neutrophil precursors are comprised of blast cells, promyclocytes, neutrophilic myelocytes, and neutrophilic metamyelocytes. Serum-free, animal protein-free cells suspensions are provided wherein the cellular component comprises at least about 3%, preferably greater than 8% megakaryocyte precursors. Also provided are serum-free, animal protein free cell suspensions wherein the cellular component comprises colony-forming cells…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.