Bacteria for preparing stable fusion proteins and methods for detecting the same

US6040141A · kind A · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Thomas Klauser · Fellbach, DE
• Joachim Ernst Kramer · Pernitz, AT
• Thomas F. Meyer · Tübingen, DE
• Johannes Pohlner · Tübingen, DE

Key dates

• Filing date: Sep 23, 1996
• Grant date: Mar 21, 2000
• Expiry date: Sep 23, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to bacteria for preparing stable fusion prons from a carrier protein and a passenger protein, the bacteria possessing the genetic marker fpt. This genetic marker permits the improved preparation of protein fusions having a destabilizing effect on bacteria. The present invention in particular relates to bacteria for preparing fusion proteins, the bacteria stably presenting the fusion proteins on their surface and possessing the markers ompT.sup.-- and dsbA.sup.-- in addition to the genetic marker fpt. Moreover, the present invention relates to the identification of bacteria, which present heterologous proteins having an affinity to a binding partner on its surface and methods for constructing vectors encoding these proteins. Finally, the present invention also relates to bacteria which stably present at least one fusion protein on their surface and possess the genetic markers fpt, ompT.sup.-- and dsbA.sup.--, and their use for instance for diagnostic purposes.