Defective DNA viral vector comprising a neural tissue-specific promoter for in vivo expression of a gene
US6040172A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 14, 1995 |
| Grant date | Mar 21, 2000 |
| Priority date | — |
| Expiry date | Feb 14, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/85
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A replication defective DNA viral vector that comprises a gene of interest encoding a protein having a demonstrated therapeutic effect on a host cell is disclosed. The vector comprises the gene in operable linkage with a neural tissue-specific promoter, which is a promoter derived from a gene normally produced in the host cell. In particular an HSV-1 vector is used. A preferred DNA defective vector is the dvHBENK which includes a promoter prepared from the rat preproenkephalin gene, the promoter being in operable linkage with the lacZ gene. The disclosed DNA viral vector is useful for achieving stable in vivo long-term expression, in particular in mammalian brain cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.