Method of sequencing nucleic acids by shift registering
US6043036A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 24, 1997 |
| Grant date | Mar 28, 2000 |
| Priority date | — |
| Expiry date | Nov 24, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
The present invention describes a method of sequencing nucleic acids in which mixtures of oligonucleotide fragments are derived from sequencing reactions using combinations of the 2',3'-dideoxynucleoside 5'-triphosphate or 3' deoxynucleoside 5'-triphosphate terminators and appropriate concentrations of four dNTPs (2'-deoxynucleoside 5' triphosphates, e.g., dATP, dCTP, dGTP, dTTP, dITP, 7-deaza-GTP). These fragments are generated by enzymatic extension of a primer hybridized to the single-stranded template DNA to be sequenced. In contrast to common slab gel sequencing methods, the method of the instant invention does not require precise alignment of the four separation sets of the terminated fragments to permit deduction of the DNA sequence. In addition, the method possesses inherent redundancy in the separations, which facilitates sequence assignment by resolving sequence uncertainties or anomalies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.