Primer walking cycle sequencing using presynthesized primers containing nucleotide analogs
US6043059A · kind A · utility
11Cited by
2References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 23, 1998 |
| Grant date | Mar 28, 2000 |
| Priority date | — |
| Expiry date | Mar 23, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for primer walking cycle sequencing of nucleic acid is provided using a presynthesized set of walking primers wherein the primers have a raised annealing temperature and/or improved annealing properties without increasing sequence complexity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.