Method for identifying genes encoding signal sequences
US6046000A · kind A · utility
3Cited by
7References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 7, 1997 |
| Grant date | Apr 4, 2000 |
| Priority date | — |
| Expiry date | Nov 7, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention involves a method of identifying nucleic acid sequences encoding signal peptide-containing proteins. The method features chimeric constructs containing a KRE9 gene that lacks a signal sequence. Yeast containing chimeric KRE9 plasmid constructs that encode signal sequences are selected based on their ability to grow on media in which sucrose is the sole carbon source.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.