Thermostable variants of human interferon-.gamma.(IFN-.gamma.)

US6046034A · kind A · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Gero Waschutza · Meinersen, DE
• Volkhart Min-Jian Li · Velbert, DE
• Bernd Otto · Hannover, DE

Key dates

• Filing date: May 11, 1998
• Grant date: Apr 4, 2000
• Expiry date: May 11, 2018

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention provides new variants of recombinant human interferon-.gamma. (rhIFN-.gamma.), vectors and host cells for their production, and therapeutic methods employing them. The variants are characterized by the substitution of one or more pairs of amino acids selected from Glu.sup.8 -Ser.sup.70, Ala.sup.18 -His.sup.112, Lys.sup.81 Leu.sup.121, and Gln.sup.49 -Leu.sup.96 by pairs of Cys residues, and optionally by the deletion of from one to ten amino acid residues from C-terminus of the native IFN-.gamma. sequence. The variants of the invention exhibit greater thermal stability and no loss of biological activity as compared to native-sequence rhIFN-.gamma..