Method for producing glycoconjugates
US6046040A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 28, 1998 |
| Grant date | Apr 4, 2000 |
| Priority date | — |
| Expiry date | Jul 28, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/18
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for producing glycoconjugate, which comprises the steps of: PA1 (i) binding a sugar residue to the side chain of a water-soluble polymer via a linker having a selectively cleavable linkage to give a primer, and bringing said primer into contact with an immobilized glycosyltransferase in the presence of a sugar nucleotide, to transfer a sugar residue of said sugar nucleotide to the sugar residue of said primer, PA1 (ii) elongating a sugar chain by transfer of plural sugar residues by repeating the step (i) at least once, PA1 (iii) removing, where necessary, a by-produced nucleotide or an unreacted sugar nucleotide, and PA1 (iv) repeating the steps (i)-(iii) where necessary and releasing the glycoconjugate by selectively cleaving the cleavable linkage in the linker, from the above-mentioned primer connecting the sugar chain elongated by the transfer of plural sugar residues, PA1 and a method for producing a sphingoglycolipid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.