Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
US6048690A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 14, 1997 |
| Grant date | Apr 11, 2000 |
| Priority date | — |
| Expiry date | May 14, 2017 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH10K85/761
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for electronic perturbation of fluorescence, chemilluminescence and other emissive materials provide for molecular biological analysis. In a preferred method for hybridization analysis of a sample, an electronic stringency control device is used to perform the steps of: providing the sample, a first probe with a fluorescent label and a second probe with a label under hybridization conditions on the electronic stringency control device, forming a hybridization product, subjecting the hybridization product to an electric field force, monitoring the fluorescence from the hybridization product, and analyzing the fluorescent signal. The label preferably serves as a quencher for the fluorescent label. In yet another aspect of this invention, a method for achieving electronic fluorescence perturbation on an electronic stringency control device comprising the steps of: locating a first polynucleotide and a second polynucleotide adjacent the electronic stringency control device, the first polynucleotide and second polynucleotide being complementary over at least a portion of their lengths and forming a hybridization product, the hybridization product having an associated environment…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.