Method for cloning and producing the DraIII restriction endonuclease
US6048719A · kind A · utility
2Cited by
4References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 22, 1999 |
| Grant date | Apr 11, 2000 |
| Priority date | — |
| Expiry date | Jan 22, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the recombinant DNA which encodes the DraIII restriction endonuclease modification methylase, and the production of DraIII restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 vector which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 vector which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.