Selectable marker for development of vectors and transformation systems in plants
US6048730A · kind A · utility
6Cited by
4References
17Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 19, 1990 |
| Grant date | Apr 11, 2000 |
| Priority date | — |
| Expiry date | Sep 19, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/12
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses functional and selectable micro-Ti plasmids. The hygromycin phosphotransferase (aphIV) gene from Escherichia coli was inserted between the 5' promoter and associated amino terminal region-encoding sequence of an octopine synthetase gene and the 3' terminator signal sequence of a nopaline synthetase gene. These constructs were assembled between T-DNA border fragments in a broad-host-range vector and used to create antibiotic-resistant plant cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.