Methods for screening for inhibitors of the transcription-enhancing activity of the X protein of hepatitis B virus
US6051373A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Dec 7, 1994 |
| Grant date | Apr 18, 2000 |
| Priority date | — |
| Expiry date | Dec 7, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2730/10122
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for screening for inhibitors of Hepatitis B Virus pX activity. The method involves contacting a test compound with (I) the pX protein of HBV, (ii) a transcription factor comprising the bZIP domain, or fragments that comprise a minimal a bZIP domain, and (iii) an oligoduplex comprising a target DNA sequence of the transcription factor to form a test mixture. After incubating the test mixture under appropriate conditions and for a sufficient time to allow pX-mediated dimerization and DNA binding of the transcription factor to occur, the level of DNA binding of the transcription factor in each test mixture is determined. A test compound is considered to be any compound that causes a decrease in the level of DNA binding in the test mixture relative to the level of DNA binding in control mixtures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.