Method of detecting p53-specific antibodies

US6051384A · kind A · utility

Assignee

• DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES ÖFFENTLICHEN RECHTS · DE

Inventors

• Hanswalter Zentgraf · Heidelberg, DE
• Peter Schranz · Durmersheim, DE
• Martin Volkmann · Heidelberg, DE
• Claudia Tessmer · Hillstett, DE
• Ralf Klein · Oberkirch, DE

Key dates

• Filing date: May 30, 1994
• Grant date: Apr 18, 2000
• Expiry date: May 30, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/975
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method of detecting p53-specific antibodies in body fluids wherein support-bound p53 and/or support-bound fragments thereof comprising binding regions for p53-specific antibodies are incubated with body fluids and the specific antibodies (a) bound to p53 and/or said fragments are allowed to react PA1 with labelled antibodies (b) directed against antibodies (a), or PA1 with unlabelled antibodies (b) while the latter are allowed to react with labelled antibodies (c) directed against antibodies (b), with the labelling being non-radioactive in either case. The invention further relates to a kit useful therefor. Also, the invention relates to p53 fragments and DNA sequences coding for them, said fragments comprising binding regions for p53-specific antibodies, as well as to methods of preparing them.