Production of C-terminal amidated peptides from recombinant protein constructs

US6051399A · kind A · utility

Assignee

• Bionebraska, Inc. · US

Inventors

• Jay Stout · Lincoln, US
• Bruce E. Partridge · Lincoln, US
• Dennis Henriksen · Lincoln, US
• Barton Holmquist · Lincoln, US
• Fred W. Wagner · Walton, US

Key dates

• Filing date: Sep 19, 1997
• Grant date: Apr 18, 2000
• Expiry date: Sep 19, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/75
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for the production of C-terminal amidated recombinant peptides is provided. The method employs a recombinant protein construct having multiple copies of a target peptide linked by intraconnecting peptides. The intraconnecting peptides permit the multicopy construct to be selectively reacted to produce product peptides having a C-terminal .alpha.-carboxamide. A recombinant gene containing a DNA sequence coding for the recombinant protein construct and an expression cassette, an expression vector and a transformed cell including the recombinant gene are also provided.