Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US6051409A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 23, 1996 |
| Grant date | Apr 18, 2000 |
| Priority date | — |
| Expiry date | Sep 23, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8207
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides an improved method for achieving stable integration of an exogenous DNA fragment in intact form into the genome of a eukaryotic cell, particularly a plant cell. The method comprises providing the exogenous DNA together with one or more proteins which promote integration of the exogenous DNA to the eukaryotic cell targeted for transformation, wherein the proteins are provided in the form of a chimeric gene or translatable RNA capable of expression in the eukaryotic cell. The method is particularly applied to plant cells to achieve stable integration of an exogenous DNA fragment bounded by T-DNA borders in intact form using integration-promoting proteins derived from Agrobacterium. Transgenic cultures, tissues and whole organisms, particularly transgenic plants, can be generated from cells transformed according to the method of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.