Vectors for transforming CHO cells

US6051426A · kind A · utility

Assignee

• Institut Pasteur, Institut National de la Sante et de la Recherche Medicale and Centre National de la Recherche Scientifique · FR

Inventors

• Elaine Sobczak · Paris, FR
• Yves Malpiece, deceased · Amiens, FR
• Marie-Louise Michel · Paris, FR
• Pierre Tiollais · Paris, FR
• Rolf E. Streeck · La Celle-Saint-Cloud, FR

Key dates

• Filing date: Feb 6, 1995
• Grant date: Apr 18, 2000
• Expiry date: Feb 6, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/108
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention concerns a composition useful for the manufacture of vaccines containing particles having the immunogenic properties characteristic of the antigen HBsAg, these particles being more particularly characterized by the fact that the particles equally contain a receptor for polymerized human albumin. They are obtained by transformation of human or animal cells by a vector containing a DNA sequence coding for the S and pre-S regions of a genome of viral hepatitis B, this DNA sequence being placed under the direct control of a promoter permitting the effective transcription of the sequence in the human or animal cells transformable by the vector.