Process for typing of HCV isolates
US6051696A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 19, 1998 |
| Grant date | Apr 18, 2000 |
| Priority date | — |
| Expiry date | Mar 19, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6834
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a process for genotyping any HCV isolate present in a biological sample, previously identified as being HCV positive, and for classifying said isolate according to the percentage of homology with other HCV isolates, comprising the steps of: PA1 contacting said sample in which the ribonucleotides or deoxyribonucleotides have been made accessible, if need be, under suitable denaturation, with at least one probe from about 10 to about 40 nucleotides, with said probe being liable to hybridize to a region being in the domain extending from nucleotide at position -291 to nucleotide at position -66 of the 5' untranslated region of one of the HCV isolates represented by their cDNA sequences, with said numbering of position beginning with the first ATG codon of the open reading frame encoding the HCV polyprotein, br with said probe being complementary to the above-defined probes, PA1 detecting the complexes possibly formed between said probe and the nucleotide sequence of the HCV isolate to be identified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.