Dyes designed for high sensitivity detection of double-stranded DNA
US6054272A · kind A · utility
16Cited by
9References
13Claims
0Family size
Assignee
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Key dates
| Filing date | Nov 7, 1997 |
| Grant date | Apr 25, 2000 |
| Priority date | — |
| Expiry date | Nov 7, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.